Helicobacter pylori virulence genes detected by string PCR in children
نویسندگان
چکیده
25 The accuracy of a nested PCR in gastric DNA obtained by string test for the diagnosis of 26 H. pylori infection in asymptomatic children was 94.0%. The cagA-positive toxigenic vacA 27 s1m1 strains were the most prevalent indicating that this population is early colonized by the 28 strains associated with gastric cancer. 29
منابع مشابه
Helicobacter pylori virulence genes detected by string PCR in children from an urban community in northeastern Brazil.
The accuracy of a nested PCR in gastric DNA obtained by a string test for the diagnosis of Helicobacter pylori infection in asymptomatic children was 94.0%. The cagA-positive toxigenic vacAs1m1 strains were the most prevalent strains, indicating that this population is colonized early by the strains associated with gastric cancer.
متن کاملPrevalence of Pathogenic Genes cagA and vacA of Helicobacter pylori Isolated in Patients with Digestive Disorders
Background and Aims: Helicobacter pylori is the main cause of various gastroduodenal diseases. It is estimated that app roximately, more than half of the adult population in developed countries and 90% of people in developing countries infected with H. pylori. H. pylori infection may be related to Genetic of virulence factors and environmental factors. The aim of this study was to assess of fre...
متن کاملUse of the noninvasive entero-test in the detection of Helicobacter pylori in children in an endemic area in Colombia.
BACKGROUND AND OBJECTIVE Gastric infection with Helicobacter pylori (H pylori), a strong risk factor for gastric cancer, is highly prevalent in children residing in the Colombian Andes. We aimed to validate the use of the Entero-test to culture and genotype H pylori strains from asymptomatic Colombian children. METHODS Children (ages 10-15 years, n = 110, 80 of which were H pylori positive by...
متن کاملPCR-based genotyping of Helicobacter pylori of Gambian children and adults directly from biopsy specimens and bacterial cultures
BACKGROUND Helicobacter pylori is an important agent of gastroduodenal disease in Africa and throughout the world. We sought to determine an optimum method for genotyping H. pylori strains from children and adults in The Gambia, West Africa. RESULTS Virulence genes were amplified in 127 of 190 cases tested (121 adults and 6 children); each of 60 bacterial cultures, and 116 from DNA extracted ...
متن کاملThe genetic diversity of Helicobacter pylori virulence genes is not associated with gastric atrophy progression.
Atrophy of the gastric mucosa is a precursor of intestinal-type gastric cancer, and Helicobacter pylori infection causes atrophic gastritis. The aim of this study was to determine whether the genetic diversity of H. pylori virulence genes is associated with the development and progression of gastric atrophy in humans. We isolated and cultured H. pylori strains from patients with gastric ulcer a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2012